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ACTA NEUROPHARMACOLOGICA››2013,Vol. 3››Issue (4): 1-7.

Effects of Morroniside on the expression of CyclinD1 and Cdk6 in Hippocampus of Rats with Focal Cerebral Ischemia/Reperfusion Injury

LIU Ting-ting, SUN Fang-ling, AI Hou-xi, ZHANG Li, WANG Wen

Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing Geriatric Medical Research Center, Beijing 100053, China

Online:2013-08-26Published:2014-06-27
Contact:王文，男，教授，博士，研究生导师；研究方向：神经药理，中药药理； E-mail：lzwwang@163.com
About author:刘婷婷，女，硕士研究生；研究方向：神经药理，中药药理；E-mail：liuting_416@126.com
Supported by:

1.“重大新药创制”科技重大专项（2012ZX09102-201-016）；2.国家自然科学基金（81173575）；3.北京市自然科学基金（7062031）；4.北京市教委科技创新平台项目（111219）；5.2011年北京市卫生系统高层次卫生技术星空娱乐app最新版计划项目（2011-3-097）；6.第52批中国博士后基金（2012M520330）；7.北京市博士后科研活动经费（106455）

Abstract

Abstract:Objective: To explore the effects of morroniside on key cell-cycle regulator proteins in a rat model of focal cerebral ischemia-reperfusion.Methods: After the model of middle cerebral artery occlusion (MCAO) was established, 15 Sprague-Dawley rats were randomly assigned into sham group, model group, morroniside low dose (30 mg·kg-1) group, morroniside middle dose (90 mg·kg-1) group and morroniside high dose (270 mg·kg-1) group. Immunohistochemistry staining was used to measure the expression of CyclinD1 and Cdk6 in ischemic ipsilateral hippocampus.Results:Compared with sham group, the expression of CyclinD1 and Cdk6 were significantly increased in vehicle-treated ischemic-reperfusion rats. However, the expression of CyclinD1 and Cdk6 was significantly reduced by treatment of morroniside at doses of 90 mg·kg-1 and 270 mg·kg-1 after ischemia-reperfusion.Conclusion: Morroniside might play a role of neuroprotection by downregulating the expression of CyclinD1 and Cdk6 after cerebral ischemia-reperfusion.

Key words:morroniside,cerebral ischemia/reperfusion,cell-cycle regulator,neuroprotective

CLC Number:

R743.3

LIU Ting-ting, SUN Fang-ling, AI Hou-xi, ZHANG Li, WANG Wen.Effects of Morroniside on the expression of CyclinD1 and Cdk6 in Hippocampus of Rats with Focal Cerebral Ischemia/Reperfusion Injury[J]. ACTA NEUROPHARMACOLOGICA, 2013, 3(4): 1-7.

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Effects of Morroniside on the expression of CyclinD1 and Cdk6 in Hippocampus of Rats with Focal Cerebral Ischemia/Reperfusion Injury

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