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Abstract

Abstract:

In vitro metabolism research is of great significance to elucidate the substance basis and mechanism of action of drugs. Common in vitro liver metabolism research methods include liver microsome in vitro warming method，hepatocyte in vitro warming method，liver perfusion technology，liver tissue sectioning technology，gene recombinant P450 enzyme system，etc. The liver metabolism of drugs in vitro cannot fully reflect the comprehensive metabolism of drugs in the body，and there is a difference from the actual metabolism in the body. In the future，it is necessary to combine in vivo experiments and other methods to improve the study of drug metabolism and transport in and out of the body；In vitro liver metabolism research methods such as the P450 enzyme system have high requirements on equipment，experimental operation costs，and data processing technology. Their application and promotion are still subject to certain constraints and restrictions. In the future，simple，fast，economical，and efficient scientific and technical methods and means need to be established. Because the liver metabolism of drugs in vitro has a huge role in promoting the research and development of new drugs and correctly guiding clinical co-administration，this article will focus on them.

Key words:in vitro liver metabolism,in vitro incubation of liver microsomes,gene recombinant P450 enzyme system,in vitro liver perfusion,organ tissue section method,in vitro incubation of liver cells

CLC Number:

R965

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LI Lan, ZHANG Dan-shen. Research Methods of Drug in Vitro Liver Metabolism[J]. ACTA NEUROPHARMACOLOGICA, 2020, 10(4): 10-13.

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URL:http://actanp.hebeinu.edu.cn/EN/10.3969/j.issn.2095-1396.2020.04.003

http://actanp.hebeinu.edu.cn/EN/Y2020/V10/I4/10

References

[1] 王莎，刘斯琪，丁鹏敏，等.体外模型在中药代谢研究中的应用[J]. 药学进展，2020，44(02)：119-132.

[2] 陈万平，孙翔，程鹏远. 体外药物肝代谢研究进展[J]. 第四军医大学学报，2008, 29(09)：861-863.

[3] Jiang Jian，Jarno Wolters，Simone G J van Breda，et al.Development of novel tools for the in vitro investigation of drug- induced liver injury[J]. Expert Opin Drug Metab Toxicol，2015，11（10）：1523-1537.

[4] 曹伟宇，冯斌，王晓娟. 肠道菌群/肝药酶系对天然皂苷类成分的代谢研究进展[J]. 中国药房，2016，27（28）：3999-4002.

[5] Su Jing，Jia Fang-yuan，Lu Jun-jie，et al.Characterization of the metabolites of rosmarinic acid in human liver microsomes using liquid chromatography combined with electrospray ionization tandem mass spectrometry[J]. Biomedical Chromatography : BMC, 2020, 34(4):e4806.

[6] Yang Juan，Chen Yan，Zhen Xiao-Ting，et al.Oxidative metabolism of typical phenolic compounds of Danshen by electrochemistry coupled to quadrupole time-of-flight tandem mass spectrometry[J]. Food chemistry, 2020, 315:126270.

[7] 李玥，王倩，苏慧宗，等. 丹参酮成分对胆汁酸体外羟化代谢的抑制研究[J]. 上海中医药杂志，2020，54(S1)：177-182.

[8]Takashi Isobea，Susumu Ohkawarab，Sadayuki Ochia，et al.Naringenin glucuronidation in liver and intestine microsomes of humans，monkeys，rats，and mice[J].Food and Chemical Toxicology, 2017, 111: 417-422.

[9] 解立科，田小亭，郭小珍，等.黄芩素与黄芩苷微生物和肝脏代谢异同研究[J]. 中成药, 2020, 42(7):1-8.

[10] 孙会仙，陈佳，郭磊，等. 胸腺素α1及其系列结构修饰物的体外肝匀浆稳定性评价[J].军事医学，2019，43(02)：146-149.

[11] Jan Vacek，Barbora Papoušková，Pavel Kosina，et al.Biotransformation of flavonols and taxifolin in hepatocyte in vitro systems as determined by liquid chromatography with various stationary phases and electrospray ionization-quadrupole time-of-flight mass spectrometry[J]. J Chromatogr B Analyt Technol Biomed Life Sci，2012，899（12）：109-115.

[12] 顾融融，阿基业，殷晓芹. 白藜芦醇对高糖及低糖培养的人肝肿瘤细胞株和人肝细胞株代谢组学的影响[J].中国临床药学杂志，2019，28(06)：419-425.

[13] Pang K S .Liver perfusion studies in drug metabolism and in drug toxicity[J].In:Mitchell JR ed .Drug Metabolism and Drug Toxicity .1st ed .New York ：Raven Press，1984 : 331-352 .

[14] 王佳，代百东.药物体外肝代谢研究方法[J].亚太传统医药，2008, 4(3)：48-50.

[15] 陈爱瑛，叶小弟，程敏，等. 大鼠离体肝灌流液中柠檬苦素的分析测定[J]. 中国新药杂志，2015，24(09)：991-993.

[16] 张如洪，邹军，李丹. 异甜菊醇在大鼠离体肝灌流中的药代动力学研究[J]. 中国医药导报，2013，10(30)：144-147.

[17] 刘海洋，路瑞华，李伟，等. .以离体肝灌流技术研究当归对黄药子肝毒性的保护作用[J].中医药信息，2012，29(02)：104-106.

[18] Bonnard P，Elsharkawy A，Zalata K，et al.Comparison of liver biopsy and noninvasive techniques for liver fibrosis assessment in patients infected with HCV- genotype 4 in Egypt[J]. Viral Hepat，2015，22(3)：245-253.

[19] 杨本坤，王素军，莫李立，等. 药物代谢体外模型的研究进展[J]. 广东药学院学报，2011，27(6)：649-652.

[20] Barbara J Ring，Jennifer S Gillespie，James Anthony Eckstein，et al.Identification of the hu man cytochromes P450 responsible for atomoxetine metabolism[J]. Drug Metab Disp，2002，30（3）：319.

[21] Seong Eun Jin，Hyekyung Ha，Soo-Jin Jeong，et al.Effects of Korean traditional herbal formula for common cold on the activities of human CYP450 isozymes[J]. J Korean Med，2014，35（2）：47-59.

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Research Methods of Drug in Vitro Liver Metabolism

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