In vitro metabolism research is of great significance to elucidate the substance basis and mechanism of action of drugs. Common in vitro liver metabolism research methods include liver microsome in vitro warming method，hepatocyte in vitro warming method，liver perfusion technology，liver tissue sectioning technology，gene recombinant P450 enzyme system，etc. The liver metabolism of drugs in vitro cannot fully reflect the comprehensive metabolism of drugs in the body，and there is a difference from the actual metabolism in the body. In the future，it is necessary to combine in vivo experiments and other methods to improve the study of drug metabolism and transport in and out of the body；In vitro liver metabolism research methods such as the P450 enzyme system have high requirements on equipment，experimental operation costs，and data processing technology. Their application and promotion are still subject to certain constraints and restrictions. In the future，simple，fast，economical，and efficient scientific and technical methods and means need to be established. Because the liver metabolism of drugs in vitro has a huge role in promoting the research and development of new drugs and correctly guiding clinical co-administration，this article will focus on them.