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Abstract

Abstract:Objective：We studied the anti-proliferation effects of 2’-bro-2-hydroxy-4，6，4’，5’-tetrametho-xychalcone（C30） on human neuroblastoma and its mechanism to provide preliminary experimental evidence for its potential application to the prevention and treatment of neuroblastoma.Methods：The antiproliferation effects of the compounds on tumor cells were detected by sulforhodamine B（SRB） assay. Cell cycle distributions were measured by flow cytometry. Cellular apoptosis was detected by Hoechst 33258 staining assay and flow cytometry.Results：C30 restrained the proliferative effect of SK-N-SH cells in a dose- and time- dependent manner. After treated with C30，the cell cycle of SK-N-SH was arrested in G1 phase. Hoechst 33258 staining and flow cytometry analysis revealed that petasin could induce apoptosis in SKN-SH cells.Conclusion：C30 can inhibit the proliferation of SK-N-SH，and the mechanism maybe related to changing cell cycle distribution and inducing apoptosis.

Key words:neuroblastoma,chalcone,cell proliferation,cell cycle,apoptosis

CLC Number:

R965

LIU Jun-ju，FU Dong-jun，NIU Jin-bo，ZHANG Qi，ZHANG Yan-bing.Antiproliferation Effects of 2’-bro-2-hydroxy-4,6,4’,5’-tetramethoxychalcone on Human Neuroblastoma SK-N-SH Cells[J]. Acta Neuropharmacologica, 2016, 6(1): 18-24.

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Antiproliferation Effects of 2’-bro-2-hydroxy-4,6,4’,5’-tetramethoxychalcone on Human Neuroblastoma SK-N-SH Cells

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