Abstract:Objective：Migraine mode of rats was made by subcutaneous injection（s.c）of nitroglycerin and the change of antonomic activity was researched.Methods：The migraine rat model was constructed by subcutaneous injection of nitroglycerin，and autonomic activities in 10，30，60，90 min after s.c nitroglycerin were real-time recorded by open stopping system.Results：Compared with control group，the special behaviors and symptoms，such as head scratching and shaking，increased in the migraine rats，and was most obvious within 30 min. The short movements（10，30 min） of migraine rats were not significantly changed. The movement distance in central area of model rats was significantly decreased，and the movement distance of peripheral area signifi cantly increased compared with control rats. These indicated that the s.c nitroglycerin rats exhibited anxiety symptom. Compared with control group，the movement distance and time of model group were decreased after 60 min，and with the increase in time，movement distance also gradually reduced.Conclusion：The increase of special behaviors and symptoms，such as the scratching head and shaking in migraine rats could be caused by s.c nitroglycerin，and meanwhile the model rats exhibited anxiety and impaired the autonomic activities were impaired.

Key words:nitroglycerin,migraine,motor ability,anxiety