Objective: To analyze the distribution of carbapenem-resistant pseudomonas aeruginosa (CRPA) in clinical departments, summarize the characteristics of drug resistance, and explore the antimicrobial methods. Methods: A total of 80 CRPA strains from March 2022 to February 2023 were selected as the observation group, and 80 carbapenem-resistant Pseudomonas aeruginosa strains from the same period were collected as the control group. The distribution of clinical departments, the characteristics of CRPA resistance, the positive rate of MBL phenotype and the results of gene detection were compared. Results: The detection rate of CRPA was higher in the departments of respiratory and critical care medicine, critical care medicine and general surgery, and the difference was statistically significant (P < 0.05). The resistance rate of CRPA to ticarcillin/clavulanic acid was significantly higher than that to other antibiotics. Amikacin and tobramycin were the most common antibiotics with low resistance rate. The positive rate of MBL phenotype was 92.50 % (74/80), MDR-PA accounted for 53.75 % (43/80), and XDR-PA accounted for 22.50 % (18/80). Conclusions: CRPA is more likely to occur in the departments of respiratory and critical care medicine and critical care medicine. Amikacin and tobramycin have good antibacterial effects in CRPA infection.