Objective：To investigate the effect of dexmedetomidine combined with sufentanil and dezocine patient-controlled intravenous analgesia （PCIA） on sleep quality in patients undergoing laparoscopic hysterectomy. Methods：80 patients undergoing laparoscopic total hysterectomy in our hospital from January 2020 to December 2022 were randomly divided into control group （n=40） and observation group （n=40）. PCIA was performed in both groups after operation，and continuous analgesia was performed for 48 hours. The observation group was given dexmedetomidine 5 μg·kg ^-1+dezocine 0.3 mg·kg ^-1+sufentanil 1.5 μg·kg ^-1+granisetron 3 mg，and the control group was given dezocine 0.3 mg·kg ^-1+sufentanil 1.5 μg·kg ^-1+ granisetron 3 mg. The visual analogue scale （VAS） score，effective pressing times，actual pressing times，remedial analgesia rate and adverse reactions of the two groups were recorded. The pittsburgh sleep quality index （PSQI） and athens insomnia scale （AIS） were assessed before operation，1 day and 3 days after operation. Results：The VAS score of the observation group was significantly lower comparing with the control group（P<0.05）. Compared with the control group，the effective pressing times and actual pressing times of analgesia pump in the observation group decreased （P<0.05），and the remedial analgesia rate decreased （P<0.05）. The incidence of postoperative nausea and vomiting in the observation group was significantly lower comparing with the control group （P<0.05）. The PSQI score and AIS score of the two groups were significantly higher than those before operation（ P<0.05）. Compared with the control group， the PSQI score and AIS score of the observation group were significantly lower at each time point after operation （P<0.05）. The incidence of postoperative sleep disorders in the observation group was 17.50 %，which was significantly lower than 37.50 % in the control group（P<0.05）. Conclusion：Dexmedetomidine combined with sufentanil and dezocine in PCIA after laparoscopic total hysterectomy can provide good analgesic effect，and improve postoperative sleep quality.