Differentiation of CD4+CD25-T Cells to Regulatory T Cells induced by Diosgenin in CIA Mice %0 Journal Article %D 2021 %J ACTA NEUROPHARMACOLOGICA %R 10.3969/j.issn.2095-1396.2021.02.002 %P 5-10 %V 11 %N 2 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_752.shtml} %8 2021-04-26 %X

Objective：To investigate the roles of diosgenin in the differentiation of regulatory T cells from CD4+CD25-T cells in collagen induced arthritis mice. Methods：

CD4+CD25-T cells of CIA mice isolated by immunomagnetic beads on magnetic activated cell sorting device were co-cultured with different concentrations of diosgenin（25、12.5、6.25μmol·L-1）for 48 hours. The ratio of Treg cells was tested by flow cytometry. The level of Foxp3 mRNA was measured by Real-Time PCR. The level of serum IL-10 was detected by ELISA. Results：This study separated CD4+CD25-T cells of 93.89% purity. The ratio of Treg cells，the level of IL-10 and Foxp3 mRNA were increased significantly in diosgenin groups compared with those in control group（P<0.05）. Conclusion：Diosgenin may induce the differentiation of CD4+CD25-T cells to regulatory T cells in CIA mice and up-regulate the expression of Foxp3.