Objective：To investigate the optimum extraction technology of mulberrin from Morus notabilis Schneid. Methods：Taking the content of mulberrin as an index，the effects of ethanol concentration，extraction time，solvent multiple，extraction times on the extraction efficiency of mulberrin was investigated by 4-Factor-3-Level orthogonal experiment. HPLC was used to detect the extraction efficiency of mulberrin on YMC-Pack ODS-A column （4.6 mm×250 mm，5 μm） with water- methanol （20：80） as mobile phase. The flow rate was 1.0 mL·min-1，the column temperature was 25 ℃ and the wavelength of UV detector was 268 nm. Results：The optimum extraction technology：before extraction，Morus notabilis was soaked in extraction solvent for 3.0 h，and extracted with reflux for 3 times，each extraction time was 0.5 h，and the extraction solvent multiple was 20 times. Under optimal extraction conditions，the content of mulberry in Morus notabilis Schneid was 1.510 mg·g-1. Conclusion：Ethanol concentration，solvent multiple and extraction times all had significant effects on the extraction efficiency of mulberry，and the extraction time didn’t have significant influences on the extraction efficiency of mulberry.