Objective：To investigate the application of Astragalus injection in intestinal ischemia-reperfusion liver injury in rats and its influence on the expression levels of Bax and Bcl-2. Methods：9 healthy adult male Wistar rats were randomly divided into 3 groups：Sham group，model group （IIR group） and Astragalus injection group （Astragalus group），with 3 rats in each group. The 3 groups were divided into 3 different phases（each time phase was 1 rats） according to the time of reperfusion（ 1，3，6 h）. After the end of the experiment，the expression levels of Bax and Bcl-2 in the left lobe of rat liver were taken as the experimental standard，and the expression levels of Bax and Bcl-2 in all specimens were detected by MIAS-2000 medical image analysis and immunohistochemical method，and the apoptosis index（AI） of liver tissues was detected by the TUNEL technique. Results：Compared with Sham group，the expression levels of Bax and Bcl-2 in three different phases of IIR group and Huangqi group were significantly increased （P<0.05），while those of Bcl-2/Bax and Bcl-2/Bax in three different phases of IIR group and

Astragalus group were decreased （P<0.05）. Compared with the IIR group，the reperfusion of bcl-2/Bax and Bcl-2 in the astragalus group at 1，3 and 6 h was significantly increased，while the level of Bax was decreased （P<0.05）. There was no significant difference between different phases in the same group （P>0.05）. Compared with Sham group，AI of IIR group and Huangqi group were significantly improved at different phases of reperfusion at 1，3 and 6 h （P<0.05）. Compared with IIR group，AI of astragalus group in three different phases was significantly reduced （P<0.05）. There were significant differences between IIR group and Astragalus group in different phases （P<0.05），and reperfusion was the highest for 6 h. Conclusion：IIR can lead to liver injury in rats，and the expression levels of Bcl-2 and Bax are increased. The mechanism of action of Astragalus injection may be to regulate the two expressions，alleviate the apoptosis of liver cells，and have a protective effect on the liver.