Objective：To explore the effect of folic acid on maternal separation mice.Methods：The newborn Kunming mice were divided into control group，maternal separation group and maternal separation plus folic acid group. Behavioral tests were started at 10-week old mice.Results：Compared with control group，the results shown that the movement and exploration behavior of the maternal separation group were decreased in the open field test，however，folic acid could increasethe movement and exploration behavior of mice；In the elevated plus maze test，the maternal separation group had less activity and exploration behavior，but folic acid group no effect compared with the maternal separation group；In the new object recognition experiment，the recognition rate of the maternal separation group was significantly lower than control group，shown the cognition was impaired while folic acid could markedly improve the recognition caused by maternal separation procedure. In the light and dark box test，the maternal separation mouse tended to move in the black box，while folic acid improved activity and tended to explore the light box.Conclusion：The maternal separation induced the anxiety- depression-like behaviors and abnormalities of learning and memory in adult mice，while folic acid can ameliorate ability of cognitive and anxiety-like behavior.