|星空彩票电脑版
%A GU Bing, WANG Shuo-yu, LI Hua-nan, WANG Jun, ZHANG Shui-yin %T
A Comparative Morphological Study on Early Stage Treatment of Rat Spinal Cord Contusion with Recombinant Human Erythropoietin and Methylprednisolone%0 Journal Article %D 2011 %J ACTA NEUROPHARMACOLOGICA %R %P 7-16 %V 1 %N 6 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_54.shtml} %8 2011-12-26 %X
Objective：To comparatively study the morphological changes of the spinal cord in rats by treatment with recombinant human erythropoietin (rHuEPO) and methylprednisolone (MP) at an early stage after contusion injury.Methods：Spinal cord contusion model in rats was duplicated with a BenchmarkTMstereotaxic impactor. 30 min after the procedure, animals were administrated with 5000 UI·kg-1·BW rHuEPO (i.p.) or 30 mg·kg-1·BW MP (tail intravenous injection). Morphological changes were examined by using the Harris HE staining and Luxol fast blue staining methods in combination with the immunohistochemical NeuN staining and GFAP staining.Results： One and 3 days after the contusion procedure, the number of survived neurons, the residual myelination area and the reduction of astrocytes in the bilateral anterior horn of gray matter in and 3-5 mm around the the injury center were significantly higher in MP group than in rHuEPO group. However, 14 and 28 days after the contusion procedure, the cavity area of and surrounding the spinal cord injury site and the reduction of astrocytes were significantly larger in rHuEPO group than in MP group. In addition, the number of survival neurons and residual myelination area 3, 5 mm surrounding the injury site were significantly larger in rHuEPO group than in MP group (P<0.05).Conclusion：MP (30 mg·kg-1·BW)treatment at the early stage after contusion damage (the first week) significantly improves the neuronal survival, reduces loss of the myelin sheath and inhibits the proliferation of astrocytes in rats with spinal cord injury. However, at a later stage (2-4 weeks after the injury) the effect of rHuEPO treatment was more significant.