|星空彩票电脑版
%A GU Li-hong，LI Lin %T
Effect of Cuprizone on Oligodendrocyte-associated Proteins%0 Journal Article %D 2018 %J ACTA NEUROPHARMACOLOGICA %R %P 58-58 %V 8 %N 2 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_444.shtml} %8 2018-04-26 %X Objective：To investigate the effect of cuprizone on the oligodendrocyte （OLs）related proteins. Methods: Mixed glial cells of cortices from neonatal rats（24 h after born） were primarily cultured for 7 d，and cells were purified by horizontal orbital shaking，differential adhesion and defined culture media. After three days，OLs were exposed to 25 nmol·L-1 cuprizone and further cultured for 3 days. Cells were observed by optical microscope and identified by immumofluorescence staining with MBP and DAPI. MBP，Fyn，Erk1/2 and other proteins were tested by Western Blot. Results：High purity mature OLs （specific marker MBP） were obtained. Compared to normal cells，the contents of MBP，Erk1/2 and p-Fyn （416 point） were significantly reduced in the cells exposed to Cuprizone. Conclusion: Cuprizone inhibits the expression of oligodendrocyte-associated proteins.