|星空彩票电脑版 %A ZHANG Mei-rong, WEI Shou-rong, WU Yan-chuan, SUN Fang-ling, AI Hou-xi, ZHANG Li, WANG Wen %T Effects of Phillyrin on MPP ⁺-induced Injury in SH-SY5Y Neuroblastoma Cells %0 Journal Article %D 2011 %J ACTA NEUROPHARMACOLOGICA %R %P 12-15 %V 1 %N 4 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_37.shtml} %8 2011-08-26 %X Objective：To investigate the effects of Phillyrin on 1-methyl-4-phenylpyridinium (MPP ⁺) -induced injury in SH-SY5Y neuroblastoma cells. Methods：SH-SY5Y cells were incubated with Phillyrin of different concentrations. MTT metabolic rate was measured as the cell survival rate to determine the effective concentrations of Phillyrin. After SH-SY5Y cells were incubated with Phillyrin (1，10，100 μmol·L ^-1) for 24 h prior to exposure to MPP ⁺(1 mmol·L ^-1) for 48 h，the MTT metabolic rate and LDH leakage rate were observed. Results：Phillyrin did not decrease the survival rate of SH-SY5Y cells at the concentrations from 1 to 100 μmol·L ^-1. Compared with the control group，MPP ⁺decreased the MTT metabolic rate （ P<0.001） and increased the LDH leakage rate ( P<0.001) of SH-SY5Y cells. However，Phillyrin significantly increased the cell activity ( P<0.05) and decreased LDH leakage rate of SH-SY5Y cells with MPP ⁺-induced injury. Conclusion：Phillyrin protects SH-SY5Y cells against MPP ⁺-induced cytotoxicity.

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