|星空彩票电脑版
%A ZHAO Nan，ZHANG You-zhi，YANG Ming，WANG Yi-wen，ZHAO Yi-min，GONG Ze-hui，LI Yun-feng %T The Antidepressant Effect of the Total Flavonoids Extracted fromCottonseed and its Regulation on Hippocampal Monoamines %0 Journal Article %D 2011 %J ACTA NEUROPHARMACOLOGICA %R %P 6-11 %V 1 %N 4 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_36.shtml} %8 2011-08-26 %X
Object
iv
e：To explore the antidepressant effect of the total flavonoids extracted from cottonseed (CTN-T) and its possible mechanism.
Methods：The learned helplessness model in rats was used to evaluate the antidepressant effect of CTN-T. Using the 5-hydroxytryptophan（5-HTP）induced head twitch response test and yohimbine toxicity potentiation test in mice，the possible monoamine targets of the antidepressant action of CTN-T were detected. Moreover， high performance liquid chromatography and electrochemical detection (HPLC-ECD) was used to determine the content of monoamine and metabolites in the hippocampus of rats following CTN-T administration.
Results：Repeated administration of CTN-T (50，100 mg·kg
-1，ig，twiceper day for 4 d) significantly decreased the helplessness behavior induced by inescapable foot- shock in learned helplessness test in rats. Repeated administration of CTN-T (50，75 mg·kg
-1， ig，twice per day for 4 d) significantly increased the 5-HTP induced head twitch response in mice，while CTN-T (60 mg·kg
-1，ig，twice per day for 4 d) had a tendency to potentiate yohimbine toxicity in mice. Repeated administration of CTN-T (25~75 mg·kg
-1，ig，twice per day for 4 d)，the neurochemical assays showed that the content of 5-hydroxytryptamine (5-HT) was increased in rat hippocampus at 25 and 100 mg·kg
-1dose of CTN-T，and that the content of norepinephrine (NE) was increased at 50 and 100 mg·kg
-1dose of CTN-T. By contrast，CTN-T had no effects on the content of dopamine (DA)，epinephrine (E)，5-hydroxyindolacetic acid (5-HIAA) and the rate of 5-HT/5-HIAA.
Conclusion：CTN-T exerts antidepressant effects which may be closely related to the strengthening of the hippocampal serotonergic and noradrenergic function.