|星空彩票电脑版
%A DU Yun-guang，CAO Xin-xin，WANG Xiao-ru，WANG Shu-hua %T
Protective Effect of Vitexin on Cerebral Ischemia-Reperfusion Injury in Rats%0 Journal Article %D 2017 %J ACTA NEUROPHARMACOLOGICA %R 10.3969/j.issn.2095-1396.2017.01.002 %P 10-23 %V 7 %N 1 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_347.shtml} %8 2017-02-26 %X
Objective：This study investigated the possible mechanism of vitexin in cerebral ischemia-reperfusion （CIR） model rats.
Methods：Middle cerebral artery occlusion （MCAO） model was established in this study. This experiment was randomly divided into sham group，model group，model+edaravone group （3.24 μmol·kg-1），and different concentrations of vitexin groups （1.62，3.24，6.48 μmol·kg-1）. After 1 h of reperfusion，the rats were intraperitoneally injected for one and three days. Triphenyltetrazolium chloride （TTC） was used to measure brain infarct volume of rats，the water content of brain tissue was measured through wet-dry weighting method，methane dicarboxylic aldehyde （MDA） content，superoxide dismutase （SOD） activity and reactive oxygen species （ROS） were determined by kit methods，the positive cells of aquaporin-4 （AQP-4） were detected by immunohistochemistry，the mRNA levels of Tolllike receptor 4 （TLR4） and nuclear factor κB （NF-κB） mRNA were detected by RT-qPCR，tumor necrosis factor α（ TNF-α） content was measured by ELISA，the cell morphology of rats was observed by HE staining.
Results：Compared to the sham group，model group showed significant
infarcts and increased brain water content. Compared to model group，vitexin decreased cerebral infarct volume and brain water content，increased SOD activity，and reduced the expressions of inflammatory factors significantly，improved cell morphology and the effects were dose dependent. Moreover，the protective effect of vitexin after 3 d treatment was better than that after 1 d. Compared to edaravone group，the effects of vitexin groups （1.62，3.24 μmol·kg-1） were lower （P<0.05，P<0.01），but showed similar effect of vitexin group at concentration of 6.48 μmol·kg-1.
Conclusion：Vitexin could protect CIR injury through attenuating oxidative stress and inhibiting the inflammatory response.