|星空彩票电脑版 %A ZHENG Yi-xin, XU Jie %T Effects of FK506 and FK1706 on Immunosuppression and Axonal Regeneration in Vitro: a Comparative Study%0 Journal Article %D 2016 %J ACTA NEUROPHARMACOLOGICA %R 10.3969/j.issn.2095-1396.2016.04.002 %P 13-18 %V 6 %N 4 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_322.shtml} %8 2016-08-26 %X Objective：To test the different effects of FK506 and FK1706 on immunosu-ppression in peripheral blood mononuclear cells and nerve regeneration in SH-SY5Y，to make sureFK1706 decrease immunosuppression while promoting nerve regeneration. Methods：Different doses of FK506 and FK1706 intervention in peripheral blood mononuclear cells were divided into nine groups：control group，different dose of FK506 group （0.1，1.0，10.0，100.0 nmol ·L -1 ），different doses of FK1706 group （0.1，1.0，10.0，100.0 nmol·L -1 ） and cell proliferation were evaluated by MTT while IL-2 levels in PBMC were evaluated by ELISA. Different dose of FK506 and FK1706 intervention SH-SH5Y cells，divided into nine groups：sham group，different concentrations of FK506 group （0.1，1.0，10.0，100.0 nmol·L -1 ），different concentrations of FK1706 group （0.1，1.0，10.0，100.0 nmol·L -1 ）. The number of cells，changes in synaptic cell body diameter and the axonal length were observed by the inverted microscope. Results：FK506 at the dose of 10.0 nmol·L -1 and 100.0 nmol·L -1 induced cell death in PBMCs. The dose of 0.1 nmol·L -1 and 1.0 nmol·L -1 reduced the cell proliferation and IL-2 secretion in concentration-and time-dependent manners （P<0.05）. FK1706 induced the decrease of PBMC proliferation and IL-2 secretion. Compared to the control group，0.1 nmol·L -1 was not statistically significant，1.0 nmol·L -1 ，10.0 nmol·L -1 ，100.0 nmol·L -1 showed after the increase of concentration and the
prolongation of the action time，the inhibitory effects of FK1706 on the cell proliferation and IL-2 secretion were signifi cantly enhanced （P<0.05），but the immunosuppression compared to the same dose of FK506 was signifi cantly attenuated （P<0.05）. Compare to the sham group，both FK506 and FK1706 could effectively promote neurite outgrowth in SH-SY5Y cells （P<0.05），there was no signifi cant difference between the two drugs （P>0.05）. Conclusion：FK1706 decrease immunosuppression while promote nerve regeneration，which may be a benefi cial strategy for nerve injury.

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