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%A LI Shuang, WANG Xiao-qin, GUO Chun-yan, ZOU Yu-an %T
Effect of Low Level of DMSO on Cell Survival Rate and Amino Acid Metabolism in SH-SY5Y Cells%0 Journal Article %D 2015 %J ACTA NEUROPHARMACOLOGICA %R %P 8-13 %V 5 %N 4 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_268.shtml} %8 2015-08-26 %X
Objective: The main objective of this article is to estimating the effect of dimethyl sulfoxide (DMSO) on livability and amino acid metabolism of SH-SY5Y cells.
Methods: SH-SY5Y cells were divided into normal group and DMSO group. The DMSO group was exposed to 0.0125% DMSO in different time (24, 48 h) to investigate amino acid transform which was associated with cell damage.SH-SY5Y cell livability was determined by MTT as well as amino acids content was determined by high performance liquid chromatography.
Results: Compared with the normal group, DMSO group`s 7 amino acids (glutamic acid, glutamine, glycine, proline, phenylalanine, isoleucine, leucine) decreased significantly after SH-SY5Y cells were treated with DMSO for 24 h (P<0.05),and 12 amino acids (glutamic acid, glutamine, glycine, proline, phenylalanine, isoleucine, leucine, serine, histidine, lysine, alanine, tryptophan) decreased significantly after treated with DMSO for 48 h (P<0.01). But there was no significant transform in cell vitality and morphological specificity.
Conclusions: Although there was no obvious change in cell vitality and morphological specificity, but amino acid concentrations had significant transform. This finding suggests that abnormal amino acid metabolism may be a potential early and causal feature of cell damage.