|星空彩票电脑版
%A Li Xiao-feng, Zhao Da-peng, Chen Shu-sha, Jing Wei, Li Xin-yi %T
The Effects of Tenuigenin on Expression of nAChRα4 and PSD95 in Alzheimer’s Disease Model Rats%0 Journal Article %D 2011 %J ACTA NEUROPHARMACOLOGICA %R %P 16-22 %V 1 %N 3 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_26.shtml} %8 2011-06-26 %X
Objective：To investigate the effects and the underlying mechanisms of Tenuigenin on Alzheimer’s disease by observing the effects of Tenuigenin(TEN) on expression of nicotinic acetylcholine receptor subunit alpha-4 (nAChRα4) and postsynaptic density 95(PSD-95) in Alzheimer’s disease model rat.
Methods：32 male Wistar rats were divided randomly into four groups: control group，model group，12.5 mg·mL
-1dose TEN group and 37.5 mg·mL
-1dose TEN group. The rat model with Alzheimer’s disease was made by injecting ibotenic acid into Meynert basal nuclei of aging rat induced by D-gal.The expressions of nAChRα4 and PSD-95 in the hippocampus CA1 were measured by immunohistochemistry method.
Results：Compared with the control group，the average gray-scale values of hippocampus CA1 nAChRα4 and PSD-95 in the model group were increased significantly and the average optical density was decreased obviously (
P<0.01). Compared with the model group，the average gray-scale values in the hippocampus CA1 of 37.5 mg·mL
-1dose TEN group and 12.5 mg·mL
-1dose TEN group were obviously decreased and the average optical density was increased significantly (
P<0.05). Meanwhile，the average gray-scale value within the hippocampus CA1 of 37.5 mg·mL
-1dose TEN group was significantly less than that in 12.5 mg·mL
-1dose TEN group，but more distinct than that on the average optical density aspect (
P<0.01).
Conclusion：TEN can dose-dependently increase the expression of CA1 area nAChRα4 and PSD-95 in Alzheimer’s disease model rats，which may partly explain the beneficial effects of TEN on cognitive function.