|星空彩票电脑版
%A Cheng Yue-fang, Wang Cai-fang, Li Wen-jie, Lin Qing, Zhang Qi %T
Antiproliferation Effects of Petasin on Human Neuroblastoma SK-N-SH Cells%0 Journal Article %D 2011 %J ACTA NEUROPHARMACOLOGICA %R %P 1-6 %V 1 %N 3 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_24.shtml} %8 2011-06-26 %X
Objective:To study the antiproliferation effects of petasin on human neuroblastoma SK-N-SH cells and the possible mechanisms involved.
Methods:The inhibitory effects of petasin on proliferation of tumor cells was detected by SRB assay;
the effects ofpetasin on cell cycle arrest of SK-N-SH were measured by flow cytometry; the effects of apoptosis were examined by Hochest 33258 staining and flow cytometry and the
phosphorylationof ERK1/2 of SK-N-SH
was determined bywestern blotting.
Results:Compared to that of other cancer cell lines such as SGC7901, SPC-A-1, SMMC 7721, Ec9706, SK-N-BE (2) and U251, the proliferation of SK-N-SH cells were strongly inhibited by petasin. After treated with petasin, the cell cycle of SK-N-SH arrested in G0/G1 phase, and there was no apoptosis detected either by Hoechst 33258 staining or by flow cytometry analysis. Finally, the phosphorylation of ERK1/2 was decreased by pretreatment with petasin in a dose-dependent way.
Conclusion:Petasin is a potential anti-tumor of natural compounds, and shows the strong antiproliferation effect on SK-N-SH cells without inducing apoptosis. The inhibition of ERK1/2 phosphorylation might be involved in the antiproliferation effects of petasin.