|星空彩票电脑版
%A MA Jian-peng, CHANG-Qing, XUE-Qian, ZOU Yu-an %T
Effect of Kangnaoye on Expression of VEGF, BDNF, MMP-9 after Cerebral Ischemia Reperfusion in Rats%0 Journal Article %D 2014 %J ACTA NEUROPHARMACOLOGICA %R %P 16-21 %V 4 %N 2 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_205.shtml} %8 2014-04-26 %X
Objective: To observe the effect of Kangnaoye pretreatment on the expression of vascular endothelial growth factor (VEGF), brain derived neurotrophic factor (BDNF),matrix metalloproteinase-9 (MMP-9) in rats with cerebral ischemia-reperfusion injury, and further to explore the neurovacular unit protective mechanism of Kangnaoye against the injury of cerebral ischemia-reperfusion.
Methods: Male Spraque-Dawley rats were randomly divided into: Sham operation group，ischemia-reperfusion group，Kangnaoye 28.6，14.3，7.15 g·kg -1·d-1 dose group (intragastric administration, 7 d). Improved Zea Longa method was used to prepare cerebral ischemia-reperfusion model of rats. Reperfusion after ischemia for 2 h（rats were sacrificed respectively after reperfusion 6 h，12 h，24 h，72 h，7 d）. TTC staining method was used for observing the infarct area of rat brain; Immunohistochemical method was used for observing the expression of VEGF, BDNF, MMP-9 in rats brain tissue.
Results: The comparison of the infarct area between the groups 24h after reperfusion ischemic found that the Kny High, Middle dose group significantly decreased the infarct area (P<0.05), along with the unregulated expression of VEGF,BDNF（P<0.01）. The expression of MMP-9 was significantly decreased as compared to model group（P<0.01）.
Conclusion: Kangnaoye promoted the expression of VEGF, BDNF in brain tissue, inhibited the expression of MMP-9, decreased the infarct area and protected neurovascular unit against cerebral ischemia reperfusion injury.