|星空彩票电脑版
%A WEN Zhi-ling, WANG Zhen-zhen, HE Wen-bin, CHEN Nai-hong %T
Protective Effect of Kaixin Powder Containing Serum on Corticosterone Induced CTX TNA2 Cells Injury%0 Journal Article %D 2014 %J ACTA NEUROPHARMACOLOGICA %R %P 1-5 %V 4 %N 6 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_171.shtml} %8 2014-12-26 %X
Objective: To observe the effects of Kaixin powder containing serum on corticosterone induced injury of rat cortical astrocyte CTX TNA2 cell line.
Methods: Firstly, SD rats were orally administered with Kaixin powder, positive drug fluoxetine (10 mg·kg-1) and solvent control in order to obtain corresponding serum. CTX TNA2 cell line injured by corticosterone was used to establish depression model in vitro and cell activity was detected with MTT. Then we tested whether Kaixin powder play a neuroprotective role through PI3K signaling pathway in this cell model with the inhibitors of this pathway and detected by western blotting analysis.
Results: According to our data, when the corticosterone concentration reached 200 μM, the cell viability inhibition rate was stabilized at 60% with a well concentration dependent. Kaixin powder containing serum could significantly improve the survival rate of corticosterone injured CTX TNA2 cells. The western blotting results also showed that Kaixin powder containing serum could reverse the reduction of PI3K and AKT1/2 protein expression induced by corticosterone. However, after given the inhibitors of PI3K pathway, the protective effect of Kaixin powder was reversed.
Conclusion: Kaixin powder containing serum has obvious protective effect on corticosterone injured CTX TNA2 cell line, and the mechanism may be associated with nerve cell protective related PI3K signal transduction pathway.