|星空彩票电脑版
%A TIAN Hui, WANG Qian, MEI Yan-fei, ZHANG Dan-shen, XUE Gui-ping %T
Application of Uniform Design-High Throughput Screening in Research of Vascular Endothelial Cells Protective Effect of Radix Salviae Miltiorrhizae Hydrosoluble Constituents%0 Journal Article %D 2014 %J ACTA NEUROPHARMACOLOGICA %R %P 1-10 %V 4 %N 3 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_168.shtml} %8 2014-06-26 %X
Objective: To evaluate the application of uniform design-high throughput screening technology in the study of the active ingredient of traditional Chinese medicine, vascular endothelial cells protective effect is detected by multifactorand multilevel and uniform design-high throughput screening method of Radix Salviae Miltiorrhizae hydrosoluble constituents.
Methods: We performed uniform design compatibility study on seven Radix Salviae Miltiorrhizae hydrosoluble constitutents, each of which at seven concentration levels (1×10-4～1×10-10mol·L-1) and their protective effect is assessed by the damage of vascular endothelial cells injured by H2O2 and serum deprivation.
Results: After high-throughput screening and rescreening, the best Radix Salviae Miltiorrhizae hydrosoluble combination were obtained with A2 [cell survival ratio is (70±4)%] and B4 [cell survival ratio is (76±3)%]. Different combination samples were identified with different vascular endothelial cells model which was damaged by H2O2 and serum deprivation .
Conclusion: Uniform design compatibility and the traditional Chinese medicine compound compatibility have similarities．Uniform design-high throughput screening technology is a reliable technology for massive potency screening of active ingredients of traditional Chinese medicine and compounds.