|星空彩票电脑版
%A JIN Can, ZHANG Dan-shen %T
Screening the Optimal Combination Ratio of Active Radix Salviae Miltiorrhizae Ingredients for Their Antioxidant and Hippocampal Neurons Protection Effects Using Uniform Design-High Throughput Screening Technology%0 Journal Article %D 2013 %J ACTA NEUROPHARMACOLOGICA %R %P 8-14 %V 3 %N 3 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_160.shtml} %8 2013-06-26 %X
Objective:To implement uniform design-high throughput screening (UD-HTS) technology for screening the antioxidant and hippocampal neurons protection activities of radix salviae miltiorrhizae (RSM) ingredients using multifactor and multilevel combination design.
Methods: Bioactive ingredients of RSM (7 liposoluble and 7 water-soluble) were combined with different ratios through uniform design. The bioactivity of the combination samples were assessed by anti-DPPH oxidation activity method (screening) and cytological experiment (rescreening).
Results:Two optimal combination samples of the antioxidant and hippocampal neurons protection, A13 and PA, were identified.
Conclusion: UD-HTS technology is suitable for multifactor and multilevel screening, which can provide rapid screening of optimal combinations.