|星空彩票电脑版
%A ZHANG Dan-Shen %T
The Application of Uniform Design-High Throughput Screening on Antioxidant Research of 17 Component Radix Salviae Miltiorrhiae and Compound Danshen Tablets%0 Journal Article %D 2012 %J ACTA NEUROPHARMACOLOGICA %R %P 1-17 %V 2 %N 6 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_151.shtml} %8 2012-12-26 %X
Objective: On the basis of the previous studies, and further evaluate and research the bioactive components group of Radix Salviae Miltiorrhiae (RXM) and compound Danshen tablets by multifactor and multilevel Uniform design-High throughput screening method,to find a new pattern to research traditional Chinese medicine.
Methods:By using SpectraMax M5 continuous spectrum enzyme sign reflectoscope reflector,11 of bioactive components which been confirmed with pharmacological activities of RXM and compound Danshen tablets(4 of water-solubility and 7 of liposolubility) and Compound Danshen Tablets (6 were recombined to be a new compatible group through uniform design. And the effects of the anti-oxidant microplate quantification experiment modles were detected by anti-DPPH effect,eliminating ratio of hydroxy free radical,reduction ability determination,and lipid peroxidation were established.
Results:After high-throughout screening and rescreening, better water-solubility, better water-solubility add liposolubility,and optimal combination compatibility of RXM were obtained.
Conclusion:The results indicated that effective component of RXM compatibility of different concentrations of uniform design,and with Uniform design-high throughput screening of anti-oxidant models of microplate quantification was referred to research bioactive component of compound recipe of traditional Chinese medicine.