|星空彩票电脑版
%A GAO Dong-xiao，ZHAO Ya-qun %T Production of Microencapsulated Human Retinal Pigment Epithelial Cells by the High Voltage Electrostatic Method %0 Journal Article %D 2011 %J ACTA NEUROPHARMACOLOGICA %R %P 24-27 %V 1 %N 2 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_15.shtml} %8 2011-04-26 %X
Objective: To prepare alginate-chitosan-alginate（Alginate-Chitosan-Alginate, ACA） microencapsulated Human Retinal Pigment Epithelial（hRPE） cells using high voltage electrostatic method.
Methods: The microencapsulated hRPE was prepared by using our home-made high voltage electrostatic system, and the total number and the survival rate of microencapsulated hRPE cells were studied in the culture media for 7 d.
Results: hRPE cells ACA microcapsule with diameter of （400±13） μm were obtained. the total number and the survival rate of cells had no statistically significant differences at the first 3 d. But after 7 d the total number of cells and the survival number were increased and the survival rate of cells was reduced.
Conclusion: hRPE cells ACA microcapsule can be obtained with high voltage electrostatic method; after 7 d, the microencapsulated cells grow faster.