%A XU Jian-wen, YANG Jian, YU Chang-xi
%T Changes of PTEN (Phosphatase and Tensin Homolog Deleted on Chromosome 10) Gene Level in the Hippocampus of Experimental Alzheimer’s Disease Mice
%0 Journal Article
%D 2011
%J ACTA NEUROPHARMACOLOGICA
%R 
%P 19-23
%V 1
%N 2
%U {http://actanp.hebeinu.edu.cn/CN/abstract/article_14.shtml}
%8 2011-04-26
%X <strong>Objective</strong>: To investigate the dynamic alteration of PTEN（Phosphatase and Tensin Homolog Deleted on Chromosome 10）and phospho-PTEN levels at different stages of mimic Alzheimer&rsquo;s disease（AD）mice induced by AlCl3. <strong>Methods</strong>：AD-like mouse was made by intracere-broventricular injection（icv）of 2 &mu;L of 0.5% AlCl<sub>3</sub> dissolved in artificial cerebrospinal fluid, once a day, consecutively for 5 d. Passive avoidance response test was used at d 2 and d 7, and Morris water maze test at d 23 to d 30 after the last icv injection to detect the learning and memory capabilities of mice. Western Blotting was used to detect the protein levels of PTEN and phospho-PTEN in the hippocampus of mice. <strong>Results</strong>: The results of step through passive avoidance response showed a significant retardation of the model mice from d 2 to d 7 after the last icv injection compared with that of the control group; from d 23 to d 30 after the last icv injection, Morris water maze（MWM） performance displayed a significant impairment in learning and memory ability of model mice. No significant differences on protein levels of PTEN and phospho-PTEN in the hippocampus were found between the model mice and control mice at the d 2 and d 7 time-points after the last icv injection. At the d 30 time-point after the last icv injection, the protein level of phospho-PTEN-Ser<sup>380</sup> of the model mice was significantly lower than that of the&nbsp;control group, but no significant difference about PTEN protein level. <strong>Conclusion</strong>：The phospho-PTEN protein level of hippocampus in the late development stage of mimic AD mice induced by aluminum toxicity is decreased significantly.