|星空彩票电脑版 %A HE Wei, LI Yong-min, ZHANG Hai-xia, ZHU Juan, GAO Yue %T Protective Effects of He Ying An Xin Fang on Free Radical Injury Induced by H₂O₂in Myocardium Rats%0 Journal Article %D 2014 %J ACTA NEUROPHARMACOLOGICA %R %P 17-19 %V 4 %N 1 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_136.shtml} %8 2014-02-26 %X Objective:To investigate the protective effects and mechanisms of he ying an xin fangon myocardium injury caused by H ₂O ₂. Methods:Hydrogen peroxide (H ₂O ₂) was adopted to establish the rat embryonic cardiomyocytes oxidative stress insult model , and the experiment was divided into 5 groups: cardiomyocytes were incubated by H ₂O ₂(200 μmol·L ^-1) for 2h as model group, the non- H ₂O ₂as control group, and the extraction of he ying an xin fang (500, 250, 125 mg·kg ^-1) as therapeutic groups.
The cell viabilityby 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT), content of malondialdehyde (MDA) and activity of lactate dehydrogenase (LDH) were measured. Resulds:Cell viabilityby MTT: compared with control group, cell Viabilityby MTT was decreased significantly in H ₂O ₂group ( P< 0.01), while he ying an xin fang had no significant effect on cardiac myocytes of cell viability. Content of MDA: compared with that of H ₂O ₂group, the content of MDA was decreased in he ying an xin fang groups significantly ( P< 0.05). Content of LDH: compared with that of control group, the content of LDH was decreased in he ying an xin fang groups significantly ( P< 0.05). Conclusion:He
ying an xin fang has significant protective effects on free radical injury induced by H ₂O ₂in myocardium rats, and it may be related to the inhibition of lipid peroxidation.

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