|星空彩票电脑版
%A JIA Xiao-hui, TIAN Ying-xin, TIAN Min, HAN Xiao-dong, JIA Tian-jun %T
Construction of Cpn0147 Bait Vector in Yeast Two-hybrid from Chlamydia Pneumonia Potentially Causing Neurodegenerative Diseases%0 Journal Article %D 2012 %J ACTA NEUROPHARMACOLOGICA %R %P 19-23 %V 2 %N 5 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_127.shtml} %8 2012-10-26 %X
Objective:To construct a bait vector of pGBKT7-Cpn0147 for seeking its ligands with yeast two-hybrid system.
Methods:Cpn0147 gene was amplified by polymerase chain reaction (PCR), and then cloned into pGBKT7 to construct the bait vector. These recombinant plasmids were transformed into yeast cells AH109 and Y187 to test toxic effects and self-activation activity.
Results:The pGBKT7-Cpn0147 bait vector was constructed successfully, which neither had self-activation ability nor yeast cell toxicity.
Conclusion: The constructed pGBKT7-Cpn0147 could be used as a bait vector in yeast two-hybrid system, and provided an experimental basis for further screening the interaction proteins.