%A YAN Miao, WANG Shi-rui, LI Mei
%T <strong>A Model of Cerebral Ischemia Injury in Streptozotocin-Induced Diabetic Rats</strong>
%0 Journal Article
%D 2013
%J ACTA NEUROPHARMACOLOGICA
%R 
%P 15-19
%V 3
%N 6
%U {http://actanp.hebeinu.edu.cn/CN/abstract/article_105.shtml}
%8 2013-12-26
%X <strong>Objective: </strong>To establish a reliable model of focal cerebral ischemia injury in diabetic rats, and to explore the criteria for successful modeling. <strong>Methods: </strong>Forty healthy male SD rats were randomly divided into three groups: a normal sham-operation group, a normal cerebral ischemia-reperfusion injury group and a group of diabetes combined with cerebral ischemia-reperfusion injury. Type I diabetes was induced by an intraperitoneal injection of streptozotocin (STZ, 60 mg&middot;kg<sup>-1</sup>). The body mass and the level of fasting blood glucose in each group were measured on Weeks 1 and 4 after STZ injection. A focal cerebral ischemia-reperfusion model was prepared by a thread occlusion method four weeks after STZ treatment. The scores of neurological function were assessed and the percentage of infarct volume were calculated after 30 min ischemia followed by 24 h of reperfusion. <strong>Results: </strong>The body mass (<em>P</em>&lt;0.001) was reduced and the fasting blood glucose (<em>P</em>&lt;0.001) was increased significantly in diabetic rats than those in normal rats four weeks after STZ injection. The percentage of infarct volume (<em>P</em>&lt;0.001) was increased more significantly and the brain injury appeared more severe in diabetic rats than in normal rats after reperfusion. The success rate of this model was 73%. <strong>Conclusion:</strong> In this study, we developed a model of focal cerebral ischemia injury in diabetic rats that demonstrates a high success rate and good reliability and stability.