|星空彩票电脑版
%A ZHOU Jing, ZHAO Hong, LIU Jian-sheng, YAO Su-yan, ZHENG De-yu %T
Influence of Chlorogenic Acid on Aβ25-35-induced Damage to Granular Cell in Cerebellum%0 Journal Article %D 2013 %J ACTA NEUROPHARMACOLOGICA %R %P 9-14 %V 3 %N 6 %U {http://actanp.hebeinu.edu.cn/CN/abstract/article_104.shtml} %8 2013-12-26 %X
Objective:To investigate the effects of chlorogenic acid on granular cell in cerebellum induced by amyloid β- protein -induced macrophage activation
in vitro.
Methods:The experiment was divided into blank control group, Aβ
25-35treatment group and Aβ
25-35+CHA co-processing of different time group; Blank control group: Equal amount of culture medium; Aβ
25-35treatment group: with Aβ
25-3510 μmol·L
-1; Aβ
25-35and different time of CHA treatment group; The co-cultured system of cerebellar granular cells and macrophages were cultivated for 2 days and then the 10 μmol·L
-1Aβ
25-35and 1000 μmol·L
-1CHA were added into this system for 6, 12, 24, 48, 96 h respectively. The growth phynotype of neurons and macrophages were detected by inverted phase contrast microscope; The expressions of p38MAPK, MAPKAPK-2, HSP27 protein were tested by Western blot.
Results:The numbers cerebellar granule cell of chlorogenic acid protection group was increased significantly compared with Aβ
25-35injury group. CHA could inhibite increased expression of phosphorylated p38MAPK of macrophage induced by Aβ
25-35at difference time; Decreased expression of phosphorylated MAPKAPK-2 and phosphorylated HSP-27of macrophage induced by Aβ
25-35could increased after the 1000 μmol·L
-1CHA added into the medium.
Conclusion:The protection of Chlorogenic acid for cerebellar granular cells induced by Aβ
25-35via inhibition the activation of p38MAPK signaling pathway, which decreased the expression of p38MAPK and increased the expression of MAPKAPK-2, HSP-27.